Novor

Important note: This application can be used free, but only for academic purposes such as researches.

If you're working in the genetics field, probably the concept of Peptide de Novo sequencing is not alien to you, as you might've already encountered some situations that required performing this type of analysis.
Novor is one of the applications that can come in handy in the situation mentioned above, as it provides you with several useful tools.

Helps you perform Peptide de Novo sequencing

You can turn to this application if you need a quick, efficient way to perform Peptide de Novo sequencing. You just have to adjust a series of parameters and feed a data file to the program, as the rest of the process is carried out automatically.
The default enzyme is set to Trypsin, but you can still choose your favorite instrument type between "HCD - FT" and "CID - Trap" and choose the fixed and variable modifications by ticking the corresponding checkboxes. The app packs a sample data file that you can load and test its capabilities without too much hassle.

Requires Java

It should be noted that in order to run this program on the target computer, you must install Java Runtime Environment beforehand. Otherwise, you won't be able to access it.
The application comes with a simple interface that encompasses a bunch of combo menus, a few buttons and two panes where you can load values by simply ticking checkboxes.

Portable tool

Since it is a portable application, Novor doesn't need to be installed on the target computer, as simply unpacking its archive and launching the JAR file grants you full access to its controls.
More so, you can run it from removable storage media including USB flash drives and external HDDs. It won't tamper with your Windows registry entries, nor will it create additional files or folders on your PC without your explicit permission.

Lightweight, Java-based Peptide de Novo sequencing tool

To wrap it up, if you need a quick way to performm Peptide de Novo sequencing, you might want to try Novor. It is portable, requires Java to run, comes with a simple interface and packs a sample data file.